Malachite Green Phosphate Assay Kit
This malachite green phosphate detection kit employs a simple, sensitive, reproducible, and non-radioactive method to measure inorganic phosphate in aqueous solutions. The assay is based on the malachite green and molybdate binding reaction and has a wide variety of applications, including the measurement of protein and lipid phosphorylation and phosphatase activity. The protocol presented is for the phosphate assay in a 96-well microplate format. The Malachite Green Phosphate Detection Kit can also be used with 1 mL cuvettes or 384-well plates suitable for high throughput screening.
Kit Contents for Malachite Green Phosphate Detection Kit
- Malachite Green Reagent A: Ammonium Molybdate in Sulfuric Acid 3M
- Malachite green Reagent B – Malachite green oxalate and polyvinyl alcohol
- phosphate standard
Product Specifications for Malachite Green Phosphate Detection Kit
Assay Type: Malachite Green Phosphate Detection Kit
Assay duration: 4 hours 40 minutes (after plate preparation)
Sample volume required: 50 µL
Sufficient materials: for fifteen 96-well plates*
Species: Multiple Species
Preparation and Storage
The product is shipped at room temperature. Upon receipt, store immediately at the recommended temperature below.
Store unopened products at room temperature. Do not use it after the expiration date.